DNA SEQUENCING (full length)

The traditional identification of bacteria on the basis of biochemical characteristics is generally not as accurate as identification based on genotypic methods. Comparison of the bacterial 16S rRNA gene sequence has emerged as a preferred genetic technique. 16S rRNA gene sequence analysis can better identify poorly described, rarely isolated, or phenotypically aberrant strains, can be routinely used for identification of mycobacteria, and can lead to the recognition of novel pathogens and noncultured bacteria. The rapid identification of pathogenic bacteria can be achieved from different biological samples by PCR amplification of 16S rDNA with “universal” primers followed by automated DNA sequencing.

The 16S rRNA gene sequence is about 1,550 bp long and is composed of both variable and conserved regions. The gene is large enough, with sufficient interspecific polymorphisms of 16S rRNA gene, to provide distinguishing and statistically valid measurements. Universal primers are usually chosen as complementary to the conserved regions at the beginning of the gene and at either the 540-bp region or at the end of the whole sequence (about the 1,550-bp) region.

Chelab offers the amplification and sequencing of full lenght 16S rRNA gene (~1500 bp) Click here to learn more technical details.

The service includes:
- PCR from heat lysated colony or from genomic DNA
- purification of PCR products
- two strands sequencing (6 sequences)
- BLAST homology search and identification of the unknown bacterium using GenBank Databases

Test results are available in 5 business days


PROCEDURE

1. Choose the number of samples: remember to send the sample in the Eppendorf tube (click here to learn more details) or 200 ng of genomic DNA (conc. 2,5 ng/ ml).

2. Click the order button and read carefully the following instruction

3. Fill in the apposite space the information regarding your samples (e.g.  aspect, condition...)

4. Fulfil the registration form or login with your username and password if you are already a customer

5. Pay on line with any credit card. Chelab sends you the invoice

6. Send your samples to Chelab

7. Chelab publishes your test results in your password proctected area